Device

Part:BBa_M36256:Design

Designed by: Alexandros Christodoulou and Matthew Loeun   Group: Stanford BIOE44 - S11   (2014-10-24)


Nitric Oxide ->PoPS


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 45
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 45
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 45
    Illegal AgeI site found at 727
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 502


Design Notes

We needed to find the sequence for a transcription factor sensitive to nitrate or nitrite or an intermediary in their breakdown and an associated promoter region.


Source

The activator protein and promoter region in this gene were taken from the norRVW operon in e.coli.

References